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Figure 4 | Molecular Cancer

Figure 4

From: Functional analysis of HOXD9 in human gliomas and glioma cancer stem cells

Figure 4

Silencing of HOXD9 gene induces apoptosis in U87 glioma cells. (A) Average cell division time of siRNA-treated cells was measured for 4 days starting 24 h after transfection (n = 3). *, P < 0.05. Error bars indicate ± S.D. (B) Representative images of U87 glioma cells transfected with control or HOXD9 siRNA in the time-laps cell imaging analysis of three independent experiments (C) Determination of apoptosis by flow cytometry 3 days after siRNA transfection. Early apoptosis (lower right, Annexin V+/7ADD-), late apoptosis (upper right, Annexin V-/7ADD+), necrotic cells (upper left, Annexin V-/7ADD+), and normal cells (lower left, Annexin V-/7-ADD-) from a representative experiment at least three independent experiments. (D) Average percentage of Annexin V+ cells. For the graphs, the data are complied from three independent experiments. **, P < 0.01. Error bars indicate ± S.D. (E) Analysis of caspase3/7 activity in U87 glioma cells 48 hr after siRNA treatment. For the graphs, the data are complied from three independent experiments. *, P < 0.05, **, P < 0.01. Error bars indicate ± S.D.

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