Figure 5

Effect of HOXD9 gene knockdown on the expression of apoptosis- and cell proliferation-related factors. (A) Gene expression in U87 glioma cells transfected with HOXD9 siRNA or control siRNA was determined by microarray analysis. A gene list generated by a Gene Ontology search against "Apoptosis regulator activity" and "Cell growth and/or maintenance" was clustered using a hierarchical method. The red, green, and brown scale represents the expression level of a gene above, below, or equal to the mean expression level for that gene across all samples, respectively. (-) indicates that the gene was not annotated in the NCBI gene bank list. (B) Analysis of BCL-2 and TRAIL gene expression in U87 glioma cells by qRT-PCR 2 days (BCL-2) or 3 days (TRAIL) after siRNAs transfection. Each mRNA was normalized against GAPDH mRNA and expressed relative to the normalized value for control siRNA-treated cells. For the graphs, the data are complied from three independent experiments. **, P < 0.01. Error bars indicate ± S.D.