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Figure 3 | Molecular Cancer

Figure 3

From: Decreased expression of BRCA1 in SK-BR-3 cells is the result of aberrant activation of the GABP Beta promoter by an NRF-1-containing complex

Figure 3

Exogenous GABPβ in SK-BR-3 cells restores BRCA1 proximal promoter activity and stabilizes endogenous GABPα. (a) Expression vectors for the individual GABP subunits, or both together, were cotransfected with the BRCA1 L6-pRL promoter construct in SK-BR-3 cells. (b) SK-BR-3 cells were co-transfected with the indicated FLAG-tagged GABP expression vectors. Whole cell lysates from these cells were analyzed by Western blots probed with antibodies against GABPα, GABPβ or the FLAG moiety and then reprobed with anti-γ-tubulin to control for sample loading. The arrow indicates the band corresponding to endogenous GABPα protein. Apparent molecular weight markers (kDa) are presented to the right of the panels.

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Molecular Cancer

ISSN: 1476-4598