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Figure 5 | Molecular Cancer

Figure 5

From: Decreased expression of BRCA1 in SK-BR-3 cells is the result of aberrant activation of the GABP Beta promoter by an NRF-1-containing complex

Figure 5

GABPβ promoter activity in MCF-7 and SK-BR-3 cell lines. A series of 5' deletion mutants of the GABPβ proximal promoter were prepared in the pRL-null reporter plasmid. Promoter constructs are named according to the 5' nucleotide position relative to the transcription start site with all constructs extending to nucleotide +194. The transcriptional activity of the GABPβ promoter constructs was assessed via dual luciferase assay using the pCMV-luc plasmid as an internal control. (a) Promoter activity is expressed relative to the activity of the longest construct,-1023. (b) SK-BR-3 raw Renilla light units were multiplied by a correction factor to compensate for differences in transfection efficiency and permit a comparison of the absolute promoter activity between the two cell lines (Relative Renilla light units). The correction factor was based upon the luciferase light units (LLU) of the internal control, pCMV-luc, and was calculated by dividing the mean MCF-7 LLU by the mean SK-BR-3 LLU for the longest GABPβ promoter construct,-1023.

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