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Figure 6 | Molecular Cancer

Figure 6

From: Decreased expression of BRCA1 in SK-BR-3 cells is the result of aberrant activation of the GABP Beta promoter by an NRF-1-containing complex

Figure 6

Binding complexes that form on the GABPβ promoter. (a) A series of overlapping 20-mer oligonucleotides (sequences specified by square brackets) spanning the GABPβ promoter from nucleotide -290 to -221 were prepared to examine the binding complexes that form on the promoter. The deletion constructs that border the sequence critical for promoter activity (Figure 5) are indicated (arrows, bold). (b) The 20-mer oligonucleotides were used as probes in an EMSA with nuclear extracts from MCF-7 cells. Binding complexes are indicated (Shift = S), as are non-specific binding complexes (NS) and free probe (F). (c) Gb-270 was used as a probe in an EMSA with increasing amounts of nuclear extracts (NE) from MCF-7 and SK-BR-3 cells. Binding complexes are as indicated above.

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