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Figure 1 | Molecular Cancer

Figure 1

From: Homeobox gene Rhox5 is regulated by epigenetic mechanisms in cancer and stem cells and promotes cancer growth

Figure 1

Rhox5 expression in ES cells, somatic cells and cancer cells. (A). Schematic presentation of the Pd and Pp promoter regions of the Rhox5 gene. Vertical bars represent CpG dinucletides and open and filled boxes indicated non-coding and coding exons. Three segments (BS-1, BS-2, BS-3) covering CpG dinucleotides subject to bisulfite sequencing and corresponding regions for ChIP assay (ChIP-1 and ChIP-2) were also indicated. (B). Rhox5 mRNA in representative cancer cell lines was detected by semi-quantitative RT-PCR. M: PCR marker. (C). Relative Rhox5 mRNA levels as normalized to 1.0E4 copies of GAPDH mRNA. Real-time RT-PCR was performed in triplicates and data from multiple experiments are presented as mean +/- s.d. The data for ES cells were obtained from single RT-PCR in triplicates. (D). Transcripts from either Pd or Pp were determined by promoter-specific RT-PCR. Lane M: DNA MW Markers; T: testis; H2 O: water instead of RNA was used in RT. (E). Western blot analysis of Rhox5 in germline tissues and representative cancer cell lines.

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