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Figure 5 | Molecular Cancer

Figure 5

From: Homeobox gene Rhox5 is regulated by epigenetic mechanisms in cancer and stem cells and promotes cancer growth

Figure 5

Upregulation of Rhox5 mRNA was associated with dynamic changes of H3K4 and H3K27 methylation marks in Pd region. (A). Upregulation of Rhox5 in differentiating F9 cells as induced by MS-275, RA or RA plus cAMP. The mRNA was quantified by RT-qPCR, and the level of Rhox5 mRNA in mock-treated was arbitrarily set as 1.0. (B). Occupancy of H3 K4me2, K27me3, and K9me2 marks in Pd region in mock, MS-275, and RA-treated F9 cells as determined by real-time RT-PCR. (C). Upregulation of Rhox5 mRNA in DAC or MS-275-treated cancer cells. Fold change of Rhox5 mRNA (as normalized by Gapdh ) was compared to mock-treated cells. (D). ChIP analysis of K4me2, K27me3 and K9me2 marks at Rhox5 Pd region (ChIP-1) in mock and epigenetic drug-treated EMT6 and P815 cancer cells.

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