Figure 5From: Homeobox gene Rhox5 is regulated by epigenetic mechanisms in cancer and stem cells and promotes cancer growthUpregulation of Rhox5 mRNA was associated with dynamic changes of H3K4 and H3K27 methylation marks in Pd region. (A). Upregulation of Rhox5 in differentiating F9 cells as induced by MS-275, RA or RA plus cAMP. The mRNA was quantified by RT-qPCR, and the level of Rhox5 mRNA in mock-treated was arbitrarily set as 1.0. (B). Occupancy of H3 K4me2, K27me3, and K9me2 marks in Pd region in mock, MS-275, and RA-treated F9 cells as determined by real-time RT-PCR. (C). Upregulation of Rhox5 mRNA in DAC or MS-275-treated cancer cells. Fold change of Rhox5 mRNA (as normalized by Gapdh ) was compared to mock-treated cells. (D). ChIP analysis of K4me2, K27me3 and K9me2 marks at Rhox5 Pd region (ChIP-1) in mock and epigenetic drug-treated EMT6 and P815 cancer cells.Back to article page