Figure 7
ShRNA-mediated knockdown of Rhox5 reduced cell migration and attenuated growth of CT26 cancer cells. (A). Efficiency of Rhox5 knockdown of shRNA clones with different target sequences against Rhox5. Lentiviral particles with control empty vector (CTV) or 2 different target sets (clone 48, 49) of shRNA were used to infect CT26 cells. After 3rd round of puromycin selection, total RNA was purified and analyzed with RT-qPCR and expression of relative Rhox5 mRNA was compared to the empty vector control (set as 1.0). (B). Reduced Rhox5 protein level in Rhox5 shRNA-treated clone 49 cells. Representative result of Western blot film exposed for a short time (10 seconds) was shown. Rhox5 protein in clone 49 was detected in a longer exposure. (C). Proliferation of CT26 cells stably-transfected with control or targeted shRNA at different time points in vitro. (D). Migration ability of CT26 cells stably-transfected with control or targeted shRNA. (E). Tumor volumes (left side) and tumor weight (right side) of control and targeted shRNA stably-transfected CT26 cells at day 19 after inoculation in athymic nude mice. *: p < 0.05 when compared with control groups.