Figure 5

Effect of SL0101 on MSP or MSP plus TGF-β1-induced cell migration. The monolayer of M-RON cells in DMEM with 1% FBS) were wounded with a plastic tip and then stimulated with MSP (2 nM), TGF-β1 (5 ng/ml), or both in the presence or absence of CP-1 (300 μg/ml), PD98059 (100 μM), and SL0101 (50 μM). After incubation for 16 h, cells that migrated into the open spaces were observed under microscope and photographed. Wounded areas that covered by migrated cells were calculated as previously described [35]. Data shown here are from one of two experiments with similar results. Scale bars represent 50 μm.