Skip to main content

Advertisement

Table 1 Effect of Various Small Chemical Inhibitors on MSP-Induced Spindle-Like Morphologies in RON-Expressing MDCK cells*

From: Ribosomal Protein S6 Kinase (RSK)-2 as a central effector molecule in RON receptor tyrosine kinase mediated epithelial to mesenchymal transition induced by macrophage-stimulating protein

Small molecule inhibitors Signal proteins inhibited Amount used to treat cells Cell elongation index Preventive effect (%)
Control N/A N/A 1.0 ± 0.24 None
MSP none 2.0 nM 3.12 ± 0.47 N.D
CP-1 RON 100 μM 1.05 ± 0.26 98.4%
PD98059 MEK1/2 100 μM 1.03 ± 0.31 97.1%
Wortmannin PI-3 kinase 50 μM 2.9 ± 0.41 7.1%
SB203580 p38 MAP kinase 50 μM 3.1 ± 4.6 0.6%
SP600125 JNK1,2,3 5 μM 3.07 ± 0.39 1.6%
Cay10512 NF-κB 5 μM 2.03 ± 0.33 39.4%
S31-201 Stat3 10 μM 1.25 ± 0.34 59.9%
XAV-939 Wnt/β-catenin 5 μM 2.16 ± 0.32 30.8%
SL0101 RSK 50 μM 1.09 ± 0.27 97.1%
Rapamycin FRAP/mTOR 100 nM 2.69 ± 0.25 13.8%
Vismodegib Hedgehog 1 μM 2.04 ± 0.22 34.6%
SB431542 TGF-β1 receptor 1 μM 2.97 ± 0.24 4.8%
  1. *M-RON cells (1 × 104 cells/well) in DMEM containing 1% FBS were stimulated with 2 nM of MSP in the presence of absence of individual small chemical inhibitors for 24 h. Cell morphological changes were observed under microscope and photographed with CCD camera. Cell elongation index (CEI) was determined by measuring the length of individual adherent cells among various groups. CEI from control cells was set as one. The percentages of preventive effect were calculated by comparing with as the CEI from MSP-treated cells previously described [45]