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Figure 3 | Molecular Cancer

Figure 3

From: Differential roles of Smad2 and Smad3 in the regulation of TGF-β1-mediated growth inhibition and cell migration in pancreatic ductal adenocarcinoma cells: control by Rac1

Figure 3

Inhibition of Rac1 activity potentiates TGF-β1-induced growth inhibition and expression of p21WAF1. (A) PANC-1 cells were retrovirally transduced in a stable fashion with dn Rac1 (N17 mutation) and several individual clones tested for their susceptibility to TGF-β1-mediated growth inhibition by [3H]-thymidine incorporation assay (left graph) and direct cell counting (right graph). Data represent the mean ± standard deviation from six wells (thymidine incorporation) and 3 wells (cell numbers) processed in parallel. Shown is a representative experiment each from three independent experiments. Asterisks, p < 0.001. (B) Immunoblot analysis of p21WAF1 expression in three individual PANC-1-dn Rac1 clones and control cells stimulated or not with TGF-β1 for 24 h. β-actin was used as a loading control.

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