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Figure 4 | Molecular Cancer

Figure 4

From: Differential roles of Smad2 and Smad3 in the regulation of TGF-β1-mediated growth inhibition and cell migration in pancreatic ductal adenocarcinoma cells: control by Rac1

Figure 4

Inhibition of Rac1 expression or activity mimics the effect of Smad2 silencing on basal and TGF-β1-induced cell motility. ( A) SiRNA-mediated silencing of Rac1 suppresses basal and TGF-β1-induced cell migration. PANC-1 cells were transfected twice with siRNAs to Rac1, or matched control siRNA, as described in the Method section and analysed for the cell's migratory response to TGF-β1 in RTCA cell migration assay. (B) Pharmacologic inhibition of Rac1 inhibits basal and TGF-β1-induced cell migration of PDAC cells. COLO 357 cells (60,000/well) were pretreated, or not, with 100 μM of the chemical Rac1 inhibitor NSC23766 for 1 h prior to analysis in the RTCA assay which was carried out in the presence or absence of 100 μM NSC23766. Data in (A) and (B) represent the mean ± standard deviation of four wells processed in parallel. Data are significant because of the large number of independently measured time points. Shown is a representative experiment chosen from a series of three independent experiments.

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Molecular Cancer

ISSN: 1476-4598