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Figure 5 | Molecular Cancer

Figure 5

From: Differential roles of Smad2 and Smad3 in the regulation of TGF-β1-mediated growth inhibition and cell migration in pancreatic ductal adenocarcinoma cells: control by Rac1

Figure 5

Dn Rac1 abrogates Smad2-specific transcription but enhances Smad3-specifc transcription. (A) PANC-1 cells were cotransfected with the Smad2-specific reporter plasmid pAR3-luc (+ FAST-1), or pCAGA-luc, and dn Rac1, or empty vector as indicated. 24 h after the start of transfection cells were stimulated with TGF-β1 for another 24 h and subjected to lysis. Firefly luciferase activity was measured and normalized for Renilla luciferase activity. (B) As in (A) except that cells were transfected with either control siRNA, or Smad2, or Smad3 siRNAs (as indicated) instead of empty or dn Rac1 plasmid DNAs, as outlined in detail in the Method section. Twenty-four hours after the second round of transfection cells were stimulated for another 24 h with TGF-β1 followed by lysis and dual luciferase measurements. Shown are data from one representative experiment (out of at least three performed in total), representing the normalized mean ± standard deviation of 6 wells processed in parallel. **, p < 0.01; ***, p < 0.001.

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Molecular Cancer

ISSN: 1476-4598