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Figure 1 | Molecular Cancer

Figure 1

From: Histone deacetylase turnover and recovery in sulforaphane-treated colon cancer cells: competing actions of 14-3-3 and Pin1 in HDAC3/SMRT corepressor complex dissociation/reassembly

Figure 1

Time-course studies of sulforaphane (SFN)-induced changes in cell cycle progression and histone deacetylase (HDAC) activity. Human HCT116 colon cancer cells were plated at 0.1 × 106 cells/dish and 24 h later they were treated with SFN (15 μM), or with DMSO as vehicle control (-SFN). At selected times thereafter whole cell lysates were evaluated in the (A) MTT assay, (B) ViaCount assay, (C) Guava Cell Cycle Assay, and (D) HDAC activity assay (BioMol kit), as described in Methods. Data (mean ± SE, n = 3) were from a single experiment in each case, and are representative of the findings from three separate experiments. **P < 0.01; ***P < 0.001, compared with the corresponding vehicle control.

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Molecular Cancer

ISSN: 1476-4598