Figure 5
From: Epigenetic reprogramming of breast cancer cells with oocyte extracts
Effect of epigenetic reprogramming on malignant cell phenotype. Cancer cell growth after reprogramming with AOE. (A) Proliferation of MCF-7 cells after 1, 3 and 6 days of culture in adherent conditions as measured by MTT assay. (B) Growth of MCF-7 cells in anchorage-independent conditions. The top panels show cultures stained with crystal violet and representative fields of view of the same cultures in soft agar. The bottom panel show quantification of colony number as counted under a stereomicrosope. Bar = 100 μm. * indicates P < 0.05.