Effect of epigenetic reprogramming on in vivo tumorigenicity. Macroscopic and microscopic analyses of tumour xenografts. (A) Macroscopic appearance of untreated (UN) and AOE-treated tumour xenografts (AOE) and relative tumour growth curves (* indicates P < 0.05). (B) Eosin-Haematoxylin staining of untreated tumour sections (black arrows: mitotic figures; Bar = 50 μm). (C) Number of mitotic figures for two independent tumours in untreated (UN) and AOE-treated xenografts (AOE) (* indicates P < 0.05). (D) Interstitial stroma present in AOE-treated tumours stained for collagen (blue staining; Bar = 50 μm).