Expression of PIK3CA, p-PKB/AKT and total PKB/AKT expression in FK16A cells. (A) mRNA expression for PIK3CA in primary keratinocytes (p4), immortal FK16A cells (p38) and anchorage independent FK16A cells (p99). Shown are PIK3CA levels relative to snRNP. (B) Protein expression of PIK3CA, p-PKB/AKT, total PKB/AKT and actin in FK16A cells at immortal (p50) and anchorage independent (p121) stage. Second lanes for each cell line represent samples treated with 20 μM LY294002 (Ly) to inhibit PI3-kinase signalling. P: passage.