The effect of PIK3CA overexpression in FK16A cells. (A) Protein expression of PIK3CA, p-PKB/AKT, total PKB/AKT and actin in FK16A cells (p161) transfected with cDNA encoding PIK3CA as well as PIK3CA overexpressing cells treated with LY294002. (B) Cell viability as measured 4 days post-treatment using an MTT assay. Appropriate controls were set to 100%. * Indicates a statistical significant difference.