Figure 1
From: Nuclear and cytoplasmic LIMK1 enhances human breast cancer progression
LIMK1 is detected in the cytoplasm and the nucleus of both normal mammary tissue and human breast cancer tissue. A total of 67 normal breast tissue samples and 84 breast cancer samples were analyzed for stain localization and intensity. IHCs were performed using goat polyclonal antibody that specifically recognizes the C-terminus of LIMK1. (A) IHC analysis detects mostly faint cytoplasmic LIMK1 staining in normal human mammary tissue (broken arrow). Nuclei are blue with hematoxylin counterstain, without significant LIMK1 stain (solid arrow). Scale bar represents 100 microns. (B) IHC analysis detects cytoplasmic (broken arrow) and nuclear (open arrow) LIMK1 staining in normal human mammary tissue. Nuclei are stained positive with significant LIMK1 stain (open arrow). Scale bar represents 100 microns. (C) IHC analysis detects mostly cytoplasmic LIMK1 staining (broken arrow) in human breast cancer tissue. Nuclei are blue with hematoxylin counterstain, without significant LIMK1 stain (solid arrow). Scale bar represents 100 microns. (D) IHC analysis detects cytoplasmic (broken arrow) and nuclear (open arrow) LIMK1 staining in human breast cancer tissue. Nuclei are stained positive with significant LIMK1 stain (open arrow). Scale bar represents 100 microns.