LIMK1 expression correlates with phosphorylation of FAK in focal adhesions of MDA-MB-231 cells. MDA-MB-231 cells expressing GFP-only or various GFP-LIMK1 fusions were fixed and stained with antibodies against phospho-FAK (red), and phalloidin against actin (gold). GFP fluorescence is green in this figure. Images were obtained via fluorescence microscopy (non-confocal). Cellular regions with focal adhesion structures are marked by white arrows in the phospho-FAK images. Scale bar represents 20 microns. Note: non-specific binding of secondary antibody stains nuclei red.