Anchorage-independent neoplastic lung cell line proliferation is augmented by macrophage conditioned media. A: Neoplastic LM2 or JF32 cells were cultured on 0.75% agarose in serum-free media containing 0.5% BSA (Control), or in similar media conditioned by MH-S macrophages (+ MH-S CM). B-C: LM2 or JF32 colonies from (A) were quantified under 20× magnification, and means + SD from duplicate wells plotted from three independent experiments (n = 6). D: Neoplastic LM2 or JF32 cells were cultured on ultra-low attachment culture plates, and treated as above. E-F: LM2 or JF32 cells from (D) were quantified and mean + SD of live cell numbers plotted from three independent experiments (n = 3). * P < 0.05, ** P < 0.01 and *** P < 0.001 versus control by student's unpaired t-test.