Neoplastic proliferation stimulated by both IGF-1 and MØCM is additive, but unaffected by EGF. A-D: (A) LM2, (B) JF32, (C) E10 cells and (D) E9 cells were cultured with fresh serum-free media (Con), 50 ng/mL mIGF-1 (+ IGF-1) or media conditioned by MH-S macrophages (+ MØCM). Cell number was determined by MTS and mean + SD plotted as fold-change from control. E-F: (E) LM2 or (F) JF32 cells were cultured in serum-free media containing 0.5% BSA (control), with growth factors or MØCM. G-H: (G) LM2 or (H) JF32 live cell number was determined by hemocytometer after culture with or without NVP-AEW541 (- or +, respectively), and 50 ng/mL IGF-1 or MØCM as described above. Growth stimuli were added at 0 hrs, which was 18 hrs after cell plating. Data was pooled from at least 3 independent experiments with all conditions assayed in triplicate, and mean + SD plotted as fold-change from untreated controls (normalized to 1; A-F). * P < 0.05 and ** P < 0.001 versus control (or "-, Con"), &P < 0.001 vs. the 2 ng/mL IGF-1 group and # P < 0.05, ## P < 0.001 versus MØCM single treatment by 1-way ANOVA.