Figure 1
From: Rare human papillomavirus 16 E6 variants reveal significant oncogenic potential
Gene expression of HPV16 E6 variants in NIKS as defined by reverse transcriptase polymerase chain reaction (RT-PCR), Western blot and immunofluorescence. RT-PCR detected three bands representing mRNAs of the full length and the splice variants E6I and E6II (A). With the E6 antibody used (clone 6F4) and input of 120 μg of total protein, a band of the expected size was detected in extracts of NIKS transduced with all E6 proteins except R8Q. Loading of 200 μg total protein detected a faint band (data not shown). Western with the anti-HA antibody detected all the E6 proteins (B). The micrograph shows immunofluorescence against the HA-tag epitope. E6-specific staining is present in all transduced NIKS lines (C).