Figure 3
From: Rare human papillomavirus 16 E6 variants reveal significant oncogenic potential
p53 level in HPV16 E6 variants NIKS before and after treatment with actinomycin D. (A) ELISA showing the remaining units of p53 quantified according to the number of cells after AD treatment (0.5 nM for 24 h). Mean values of at least three independent experiments are presented as average (mean) values ± standard deviation (SD). Significance was tested by a one-way ANOVA test. A P value of < 0.05 was considered significant. (B) The corresponding Western blot showed only p53-specific bands for the empty vector NIKS. After AD treatment the amount of p53 was increased approximately threefold as seen with ELISA.