Figure 1From: Inhibition of Rho-associated coiled-coil containing protein kinase enhances the activation of epidermal growth factor receptor in pancreatic cancer cellsThe effects of Y27632 and EGF on pancreatic cancer cell proliferation. (A) Panc1, KP3 and AsPc1 cells were pretreated with 3 μM Y27632 or vehicle in RPMI containing 0.3% FCS for 1 h, and then stimulated with 30 ng/ml of EGF or vehicle for 24 h. The BrdU incorporation as the % of the control (lane 1) is shown. (B) Panc1 cells were treated with the indicated doses of Y27632 or vehicle in RPMI containing 3% FCS for 48 h, and the cell viability assay was performed using the MTT cell proliferation kit I. (C) The attached cells were treated with 0.5 μg/ml of normal mouse-IgG (open circle) or anti-EGFR-neutralizing antibodies (closed circle) for the indicated periods in medium containing 3% FCS, and the surviving cells were counted using the MTT cell proliferation kit I. The results are expressed as the absorbance (OD 560 nm-OD 750 nm). All assays were done in triplicate. (*) indicates a significant difference (p < 0.05) compared with lane 1, (#) indicates a significant difference (p < 0.05), compared with lane 2.Back to article page