Expression profiling of microRNA in mismatched and Cyr61-shRNA transfected Panc-1 cells. C. The left panel shows the 28S and 18S bands of total RNA extracted from mismatched-shRNA and Cyr61-shRNA transfected Panc-1 cells. The middle and right panels depict the electropherograms of total RNA validating its integrity. D. After data set normalization, the Log2 of the relative quantification for specific miRNAs associated with inhibition of EMT and migration/invasion was plotted as a bar graph. The right panel shows a heat map comparing the average fold-changes in microRNAs with significantly higher (red) or lower (green) expression in Cyr61-shRNA transfected Panc-1 cells in comparison to mismatched-shRNA transfected Panc-1 cells as determined by miRNA Array analysis. Then the signal was globally normalized and Average Linkage Clustering was performed using a Euclidean Correlation. Clustering was visualized using the program provided by Applied Biosystems, Foster City, CA. E. Profiling of microRNAs associated with stem cell traits.