Silencing of Cyr61 promotes mesenchymal to epithelial transition (MET) and blocks in vitro migration of Panc-1 cells. A. Western blot illustrating shRNA mediated inhibition of Cyr61 expression in Panc-1 cells. B. The photomicrographs represent the morphological changes (from mesenchymal to epithelial transition, MET) in mismatched and Cyr61 silenced Panc-1 cells. The morphology of each cells are marked with yellow lines.C. The photomicrographs represent the difference in the in vitro migration of mismatched and Cyr61 silenced Panc-1 cells toward the serum for 24 hours. Results are averages ± SEM (n = 6). *p < 0.001 vs mismatched. D. Demonstration of the inhibition of paracrine action of Cyr61 on migration toward the serum for 48 h by different doses of Cyr61 neutralizing antibody. Results are averages ± SEM (n = 6). *p < 0.05 vs vehicle treated control; **p < 0.01 vs vehicle treated control and ***p < 0.001 vs vehicle treated control.