Induction of EMT and stemness by Cyr61 recombinant protein in Cyr61 negative BxPC-3 cells. A. The photomicrographs represent the morphological changes in BxPC-3 cells growing in the presence and absence of recombinant Cyr61 protein (100 ng/ml) for 48 h. The morphology of each cell is marked with black lines. B. Western blot analysis of various epithelial, mesenchymal and stem cell markers.