Flow cytometric analysis of Panc-1Cry61+ and Panc-1KOCyr61cells labeled with Vybrant DyeCycle Violet stain. (A). Side population (SP) cells were detected in Panc-1Cry61+ cells in absence of verapamil (upper left panel). Significant reduction in SP cells was observed in the presence of 50μM verapamil (upper right panel). Lower left and right panels are showing the traces of SP cells in Panc-1Cry61+ and Panc-1KOCyr61cells in the absence and presence of 50μM verapamil, respectively. Results are representative of three independent experiments. The SP cells are outlined in the figure. (B). SP cells are shown in a bar diagram as a percentage of the total cell population.