Morphological and molecular characterization of the SP cells of Panc-1. A. Panc-1 cells were stained with dye-crystal-violet (DCV)-488 Alexa and analyzed by flow cytometry. The representative images were depicted from three independent experiments. The photomicrographs shown in the right panels represent the morphology of SP and NSP cells. B. Western blots analysis of Cyr61 and various epithelial, mesenchymal and stem cell markers in SP and NSP cells.