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Figure 1 | Molecular Cancer

Figure 1

From: Irinotecan induces steroid and xenobiotic receptor (SXR) signaling to detoxification pathway in colon cancer cells

Figure 1

SXR accumulates in the nucleus after SN-38 treatment. A. LS180 and HepG2 cells were treated with 10 μM rifampicin (rif) or 10 ng/ml SN-38 for the indicated times, then nuclear and cytoplasmic extracts were prepared and subjected to WB with the indicated antibodies. B. After for 4 h exposure to 10 ng/ml SN-38, immunofluorescence experiment were performed in LS180 cells, showing SXR (green) and nucleus (red). Yellow colour indicates colocalization. C. Analysis of the cell cycle was performed by flow cytometry in LS180 cells after SN-38 exposure. D. LS180 and HepG2 cells were treated with 1 μM CPT-11 then nuclear and cytoplasmic extracts were prepared and subjected to WB as above.

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