Molecular Cancer

Table 1 Biochemical and Biological Features of Mouse Anti-RON Monoclonal Antibodies in Various Applications*

From: The monoclonal antibody Zt/f2 targeting RON receptor tyrosine kinase as potential therapeutics against tumor growth-mediated by colon cancer cells

Features	Zt/f2	Zt/#1	Zt/g9
IgG subtype	IgG2a	IgG1	IgG1
Specificity	Only human RON	Only human RON	Only human RON
Recognition	MRS	Sema	Sema
ED ₅₀	2.3 nmol/L	3.7 nmol/L	4.2 nmol/L
Reactivity to Isoforms	RON++++ RON160++++ RON165^_ RON155^_ RON110+++	RON+++, RON160++++, RON165+++, RON155++ RON110^_	RON+++, RON160+++ RON165+++, RON155++ RON110^_
Cross-reactivity with MET and EGFR	Negative	Negative	Negative
Induction of phosphorylation	+++	++	ND
Western blotting	Negative	Negative	Negative
Immunoprecipitation	++++	+++	++++
Cell surface immunofluorescence	++++	+++	+++
Immunohistochemical staining	++++	++	+++
Effect on RON expression	Down-regulation	Down-regulation	ND

* The reactivity of Zt/f2, Zt/#1, and Zt/g9 in various immune assays were determined as detailed in Materials and Methods. For immunofluorescence and immunoprecipitation, individual mAbs were used at 1 μg/ml per sample. In immunohistochemical staining, mAbs were used at 2 μg/ml. Experiments were repeated twice. The reactivity/intensity indicated by + or-was obtained by comparing to anti-RON mAb Zt/g4 [29]. MRS, maturation-required sequences; ND, not done.

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ISSN: 1476-4598

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