RECQ1 expression in human brain glioblastomas. A) Western blot analyses of surgically removed perilesional (lanes 2,4,6,8) and lesional tissues from glioblastoma multiforme (lanes 1,3,5,7) performed using the anti-RECQ1 RQ-FL antibody. Alpha-tubulin protein was used as an internal control to guarantee that the amount of proteins loaded on each well was the same. B) Representative immunostaining of RECQ1 expression in normal autoptic brain tissues (top), and perilesional and lesional glioblastoma tissues (bottom) performed using the anti-RECQ1 RQ-FL antibody and the Benchmark automatic immunostaining device (Ventana Medical System, Tucson, AZ, USA). Immunostaining was made using the anti-RECQ1 RQ-FL antibody. The nuclei positive for RECQ1 are in brown and the tissues were counterstained with hematoxylin (normal autoptic brain tissues, O.M 40X; perilesional and lesional glioblastoma tissues, O.M. 40X). B) Immunostaining for RECQ1 on human normal heart tissue counterstained with hematoxylin (O.M 20X).