Proliferative capacity of RECQ1-depleted glioblastoma cells. A) Western blot analysis of T98G, U-87 and IMR-90 cell lines transiently transfected with an siRNA against RECQ1. L indicates the Luciferase siRNA control, R the siRNA against RECQ1. B) Clonogenic assays performed in RECQ1-depleted T98G, U-87, and IMR-90 cell lines. Pictures show colonies formed after seeding 800 cells. C) Graphs showing the plating efficiencies expressed as colony forming capacity. Values represent the average ratio of the number of formed colonies to the number of cells seeded, expressed as percentage.