Figure 7From: The human RECQ1 helicase is highly expressed in glioblastoma and plays an important role in tumor cell proliferationDownregualtion of RECQ1 leads to defects in cell cycle progression and DNA synthesis in glioblastoma T98G cells. A) Flow cytometry profiles of DNA content (x-axis; propidium iodide/PI staining) versus BrdU incorporation (y-axis; anti-BrdU immunostaining) 72 h after siRNA transfection. Boxes are labeled to indicate cell cycle phases. B) The bar graph at bottom reports the percentage of G0/G1, S-phase/BrdU positive and G2/M cells in cultures that had been transfected with RECQ1 or luciferase/control siRNA pools. Results shown are the mean ± SEM from three independent experiments. C) The graph shows the cellular surviving fractions measured at different doses of hydroxyurea in control and RECQ1-depleted T98G cells. Surviving fraction values are the mean ± SEM from three independent experiments.Back to article page