Downregulation of RECQ1 triggers spontaneous γ-H2AX foci accumulation. A) Western blot analysis of T98G cell lines transiently transfected with an siRNA against RECQ1. L indicates the Luciferase siRNA control, R the siRNA against RECQ1. B) Immunostaining of endogenous RECQ1 and endogenous γ-H2AX on T98G cells after treatment with anti-RECQ1 siRNA or control siRNAs. C) The graph shows the percentage of cells that contain a defined number of γ-H2AX foci per cell.