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Figure 2 | Molecular Cancer

Figure 2

From: Inactivation of the von Hippel-Lindau tumour suppressor gene induces Neuromedin U expression in renal cancer cells

Figure 2

Neuromedin U is upregulated by hypoxia and DMOG in VHL expressing CCRCC cell lines. RCC10/VHL and RCC4/VHL cell were exposed to either 1% hypoxia in a hypoxic Galaxy R incubator (Biotech, Palo Alto, CA) or 1 mM dimethyl-oxalylglycine (DMOG; Frontier Bioscientific, Logan, UT) for 48 hours. Western analysis showed marked upregulation of both HIF-1α and HIF-2α in treated cell lines. Levels of NMU and the HIF target gene PHD3 were found to be significantly upregulated using real-time RT PCR analysis (* p < 0.01 using Student's t test).

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