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Figure 1 | Molecular Cancer

Figure 1

From: Targeting canine bladder transitional cell carcinoma with a human bladder cancer-specific ligand

Figure 1

PLZ4 binds to Canine TCC cells. A. whole cell binding assay to determine cell binding specificity of PLZ4. Cells were resuspended at 106 cells/ml and incubated with beads coated with PLZ4. If PLZ4 binds to cells in solution, bead surface would be covered with cells and exhibit a rosette pattern under the microscopy examination. This experiment was repeated 3 times for cell lines. The cell binding assay of normal canine bladder urothelial cells was repeated on 3 different dogs. a. 5637 human bladder cancer cell line; b. K9TCC-PU cell line; c. normal canine urothelial cells; d. urothelial cells from a bladder with chronic cystitis. The average diameter of the beads is 90 μm. B. Affinitofluorescence of PLZ4 peptide toward Canine TCC cell lines. Affinitofluorescence staining was performed with all five canine TCC cell lines and normal canine bladder urothelial cells of dogs euthanized for non-bladder diseases. Only fluorescence staining to normal urothelial cells (a) and K9TCC-PU cells (c) was shown. b and d showed the corresponding DAPI nuclear staining of a and c, respectively.

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