Figure 3
From: Targeting canine bladder transitional cell carcinoma with a human bladder cancer-specific ligand
Homing of PLZ4 to mouse xenograft of canine bladder cancers. Nude mice with the xenografts from TCC-PU-In at 0.5-0.8 cm in diameter were randomly selected to be injected with 100 μl (6 nmol) of pre-incubated PLZ4-biotin-SA-Cy5.5 complex or SA-Cy5.5 dye as the control. Total body imaging was performed at 0, 1, 3, 6, and 12 hours after injection. All experiments were conducted in compliance with institutional guidelines and according to the protocol (No. 12988) approved by the Institutional Animal Care and Use Committee of the University of California at Davis. A. In vivo imaging of canine K9TCC-PU-In xenografts with PLZ4. In vivo near-infrared fluorescence images were taken at different time points after injection. C: the control mouse that received SA-Cy5.5. PLZ4: the mouse that received PLZ4-Cy5.5. Red arrows point to tumor xenografts. B. Ex vivo imaging of organs and tumor xenografts for fluorescence intensity. A color bar with the fluorescence intensity in arbitrary units is shown at the bottom. C. Ex vivo quantitative analysis of fluorescence uptake in tumor xenografts. The fluorescence intensity of tumor xenografts was normalized to that of the liver and kidney of the same mouse (the normalized value of liver and kidney is defined as 1.0).