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Figure 1 | Molecular Cancer

Figure 1

From: Targeting renal cell carcinoma with NVP-BEZ235, a dual PI3K/mTOR inhibitor, in combination with sorafenib

Figure 1

NVP-BEZ235 and Sorafenib inhibit the growth of renal cancer cell lines. A, 786-0 and Caki-1 cellular growth was monitored with colorimetric MTS assay after 48 hours of treatment with NVP-BEZ235 (NVP, 1 μM) or Sorafenib (Soraf, 10 μM) either alone or in combination, or DMSO (Control). Columns, mean cell viability relative to control of four independent experiments; bars, SD. *, P < 0.05, **, P < 0.01, #, P < 0.001 compared to control, or otherwise as specified by brackets. B, NVP-BEZ235 inhibits PI3K/mTOR pathway in renal cancer cells. 786-0 and Caki-1 cells were treated with increasing doses of NVP-BEZ235 or DMSO (Control) for 4 hours. Cells were subsequently lysed and lysates were examined for phospho-Akt (Ser 473), Akt, phospho-S6 (Ser 235/236) or S6 expression level by Western blot analysis. C, 786-0 and Caki-1 cells were treated with increasing doses of sorafenib or DMSO as a control for 4 hours. Cells were processed as under panel B and analyzed for phospho-MAPK and MAPK (Thr202/Tyr204). The illustrated blots are representative of three similar experiments.

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Molecular Cancer

ISSN: 1476-4598