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Figure 5 | Molecular Cancer

Figure 5

From: ZEB1 limits adenoviral infectability by transcriptionally repressing the Coxsackie virus and Adenovirus Receptor

Figure 5

ZEB1 promotes EMT. A-C. Immunoblots. PANC-1 cells were pre-treated with TGF-β1 for two days and then transfected twice (day 0 and day 2) with ZEB1 siRNAs in the continued presence of TGF-β1. Four days after the initial transfection, cells were harvested. A. By up-regulating epithelial proteins such as E-cadherin and CAR, knockdown of ZEB1 antagonizes TGF-β-induced EMT in PANC-1 cells. Similarly, silencing of ZEB1 expression in MDA-MB-231 cells up-regulates E-cadherin and CAR, and down-regulates the mesenchymal marker fibronectin. B. PANC-1 cells were treated with TGF-β1, and harvested at the indicated time-points for analysis of the total protein fractions. C. PANC-1 cells were treated with TGF-β1 and subjected to cell fractionation. Abbreviations: C, TGF-β1 solvent control [4 mM HCl/0.1% (v/w) BSA]; UT, untransfected; Ctrl #1, siControl ON-TARGETplus Non-targeting siRNA #1 (Dharmacon); Ctrl #2, firefly luciferase-targeting siRNA; ZEB1 siRNA #1/#2, ZEB1-targeting siRNAs. Ctrl #2 and ZEB1 siRNA sequences are provided in Additional file 1 (Table S3). Chinese Hamster Ovary cells stably expressing human CAR (CHO+), or vector (CHO-) [9]. Loading controls are shown as β-actin, β-tubulin, GAPDH and PARP signals, with GAPDH as a cytoplasmic, and PARP as a nuclear marker.

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