Figure 7

Up-regulation of cell-surface CAR levels upon ZEB1 knockdown coincides with increased adenoviral infectability. A and B. Cell-surface CAR levels following ZEB1 knockdown were measured by flow cytometry on PANC-1 cells treated with TGF-β1 (A), or on MDA-MB-231 cells (B). Error bars represent standard deviations (biological duplicates). C and D. Ad-GFP uptake following ZEB1 knockdown was determined by flow cytometry measuring GFP levels (C), or by real-time PCR for virus copy number (D). Error bars represent standard deviations. A-D. CAR and GFP signals are expressed as geometric means of the fluorescence signal intensities normalized to the untreated controls (A-C), virus copy numbers as relative adenovirus fiber levels (D). Abbreviations: UT, untransfected; Ctrl #1, siControl ON-TARGETplus Non-targeting siRNA #1 (Dharmacon); Ctrl #2, firefly luciferase-targeting siRNA; ZEB1 siRNA #1/#2, ZEB1-targeting siRNAs. Ctrl #2 and ZEB1 siRNA sequences are provided in Additional file 1 (Table S3). p < 0.001 (***), p < 0.01 (**) (Student's t-test, 1-tailed, equal variance).