Src knockdown and ICAM-1 induced signalling. A. HEK 293T cells without (left panel) or with (right panel) MUC1-CFP expression were transfected with Scramble or Src targeted siRNA using Lipofectamine 2000. Following lysis and SDS-PAGE, blots were probed for Src, or as a control, tubulin. As a control, cells were treated with Lipofectamine 2000 reagent only (LP only). Cells were then assayed for ICAM-1 binding induced calcium oscillations (B) or migration through an ICAM-1 positive monolayer (C). HEK 293T, LP only condition was set to one and remaining conditions expressed as a ratio. Columns represent average oscillation factor (B) or average number of migrated cells per five fields (C) from at least three independent trials; bars, SE. Asterisk indicates pairs in the data set which are statistically different (p < 0.05).