MUC1 dimerization in human breast cancer and transfected cell lines. A. Breast cancer cell lines T47D and MCF-7, or (B) HEK 293T cells transfected with MUC1 constructs MUC1-CFP and MUCY-YFP-Fv were treated with DSS or no treatment control, lysed, ran on SDS-PAGE and probed with anti-MUC1-CD. C. HEK 293T cells were transfected with CD8/MUC1 (lane 1) MUCY-YFP-Fv (lane 2), or both (lane 3), lysed, and double transfectants immunoprecipitated with anti-CD8 (lane 4). Lysates were ran on SDS-PAGE and probed with anti-MUC1-CD. "D" and "M" indicate expected molecular weights of dimer and monomer, respectively. Whole cell lysates (WCL), PG (Protein G) + Antibody (anti-CD8) and Antibody only (Anti-CD8) lanes are included as controls.