Figure 7
ICAM-1 induced signalling in MUC1 cells after manipulation of dimerization. HEK 293T (parental), MUC1-CFP, and MUC1-CFP-Fv cells were assayed for (A) ICAM-1 binding induced calcium oscillations or (B) migration through an ICAM-1 positive monolayer, after No treatment, 1 uM AP20187D, or 1 uM AP21998M. HEK 293T (parental), no treatment condition was set to one and remaining conditions expressed as a ratio. Columns represent average oscillation factor (A) or average number of migrated cells per five fields (B) from at least three independent trials; bars, SE. Asterisk indicates pairs in the data set which are statistically different (p < 0.05).