ICAM-1 induced signalling in MUC1-CFP-Fv (AQA) transfected cells. HEK 293T (parental), MUC1-CFP-Fv and MUC1-CFP-Fv (AQA) cells were assayed for (A) ICAM-1 binding induced calcium oscillations and (B) migration through an ICAM-1 positive monolayer. HEK 293T (parental), mock (no ICAM-1) condition was set to one and remaining conditions expressed as a ratio. Columns represent average oscillation factor (A) or average number of migrated cells per five fields (B) from at least three independent trials; bars, SE. Asterisk indicates pairs in the data set which are statistically different (p < 0.05).