Figure 1

CD133 promoter is hypermethylated in prostate cancer cell lines. (A) The CD133 promoter (adapted from Shmelkov et al., 2004 [20]) (A,B,C,D1-3, E1-4 = alternative first exons; P1-P5 = promoters; Ex2 = Exon 2; ORF = open reading frame; PYRO 1-3: pyrosequencing assays; MSP1-2: MSP assays; CHIP 1-8: ChIP primers). (B) Pyrosequencing analysis of the CD133 promoter performed in prostate cell lines (PYRO 1 assay; bars = single CpG sites; n = 3 technical replicas; ± SD; black line = average of 10 CpG sites; * = p < 0.05; ** = p < 0.01; *** = p < 0.001 in an unpaired t-test).