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Figure 3 | Molecular Cancer

Figure 3

From: Regulation of the stem cell marker CD133 is independent of promoter hypermethylation in human epithelial differentiation and cancer

Figure 3

CD133 expression and methylation in prostate primary epithelial cultures. (A) qRT-PCR analysis (relative to HPRT, calibrator = Bob; N.D. = expression undetectable after 40 cycles) of CD133 expression in prostate primary epithelial cultures (RC-165N/hTERT and Bob were used as controls). Methylation analysis of the CD133 promoter in prostate primary epithelial cultures derived from BPH (B), CaP (C) and CR-CaP (D) by pyrosequencing (PYRO 1 assay; bars = single CpG sites; n = 3 technical replicas; ± SD; black line = average of 10 CpG sites; * = p < 0.05; ** = p < 0.01; *** = p < 0.001 in an unpaired t-test).

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