Figure 5

CD133 expression and methylation in prostate hierarchy. (A) qRT-PCR analysis of CD133 expression (relative to RPLP0, calibrator cells = RC-165N/hTERT; N.D. = expression undetectable after 40 cycles) and (B) pyrosequencing methylation analysis of the CD133 promoter in selected populations from prostate primary epithelial cultures (SC = stem cells; TA = transit amplifying cells; CB = committed basal cells: PYRO 1 assay; grey bars = single CpG sites; n = 3 technical replicas; ± SD; the black line = average of 10 CpG sites).