Figure 6

CD133 expression is regulated by chromatin structure in prostate cell lines and primary epithelial cultures. (A) ChIP-qPCR analysis carried out in RC-165N/hTERT, PNT2-C2, P4E6, PC3 with rabbit IgG, anti-histone H3, anti-H3K4me2, and anti-H3K27me3. qPCR primer positions are shown in Figure 1A (X axes: CD133 promoter sequence, 0 = 5'end of exon 1A; Y axes: percentage of immunoprecipitated DNA relative to input DNA; n = 3; ± SD, data shown on logarithmic scale). (B) qRT-PCR analysis of CD133 expression in P4E6 cells lines with 0.6 μM TSA for 24 and 48 hours (relative to HPRT, calibrator = Control, ± SD). (C,D) qRT-PCR analysis of CD133 expression in prostate primary epithelial cultures (PEc) treated with 0.6 μM TSA (C) or 10 mM NaBu (D) for 48 hours (relative to HPRT, calibrator = Control, ± SD, data shown on logarithmic scale).